Biotechnology and Bioengineering · 1981 · 14 citations · 10 references
Life SciencesBiotechnologyMicrobiologyBioprocess EngineeringFermentation EngineeringFermentation ScienceBatch CultureBatch FermentationFed-batch FermentationFermentation KineticsFermentation Process ControlSingle-cell Protein ProductionAutomation In FermentationAutomation Of Fermentation ProcessesCell Growth DynamicsConcentrationContinuous Fermentation ControlFermentation ControlGas MonitoringLower Bounds
Abstract Candida utilis was cultivated in a 5‐liter jar fermentor using ethanol as sole carbon source. Control of ethanol in the cultivation broth was performed by using an ethanol vapor monitoring instrument and an oxygen electrode coupled with two control circuits. By setting upper and lower bounds according to the predetermined conditions, a signal from a gas monitoring sensor switched the lower or higher bound relay governing the actuating or switch‐off of the motor; this maintained a proper concentration of ethanol in the cultivation of ethanol in the system. The growth of cells was found to be satisfactory. Cell concentration reached 64 g/liter during a 20‐hr cultivation. As the results of comparative experiments, the control mode using the gas monitoring instrument was found to be superior to that using dissolved oxygen as a controlling signal, especially at high cell concentration.
10