Nucleic Acids Research · 1978 · 49 citations · 10 references
Life SciencesMicrobiologyMolecular BiologyEnzymologyMicrobial GeneticsNucleic Acid BiochemistryEnzyme IsolationEnzyme SpecificityEnzyme-substrate RecognitionPlasmid GeneticsRestriction Enzyme TechnologySubstrate SpecificityDna Cleavage MechanismRestriction Endonuclease SpecificityRestriction EndonucleasesRestriction Enzyme DigestionRestriction-modification Systems
A site-specific restriction endonuclease has been isolated from Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequence 5'--G-G-N-C-C--3' and cleaves as indicated by the arrows. The enzyme 3'--C-C-N-G-G--5' cleaves adenovirus type 5 and lambda DNA many times, SV40 DNA 10 times and 0X174 RF DNA 2 times. Evidence is presented that the enzyme is involved in biological restriction-modification.
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Nucleotide sequence of bacteriophage G4 DNA
G N Godson, B. G. Barrell, Rodger Staden et al. · Nature · 1978 · 2.1K citations
Agarose slab-gel electrophoresis equipment
Bill Sugden, B. DeTroy, Richard J. Roberts et al. · Analytical Biochemistry · 1975 · 196 citations
A restriction endonuclease from Staphylococcus aureus
J.S. Sussenbach, C.H. Monfoort, R. Schiphof et al. · Nucleic Acids Research · 1976 · 100 citations · Full text