Nucleic Acids Research · 1976 · 100 citations · 8 references
Life SciencesMicrobiologyMolecular BiologyEnzymologyMolecular VirologyVirologyEnzyme-substrate RecognitionNucleic Acid CleavageRestriction Enzyme TechnologyDna Cleavage MechanismDouble-stranded Dna RecognitionHost Restriction-modification SystemsRestriction Endonuclease SpecificityRestriction EndonucleasesRestriction Enzyme DigestionRestriction Enzyme PurificationRestriction-modification Systems
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
8