Chemical Communications · 2010 · 38 citations · 28 references
Life SciencesBiochemistryBiotechnologyBiosensorsEnzymologyNanobiotechnologyEnzyme FunctionFluorescence SpectroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesProtein-based NanomaterialsProteolytic EnzymesFluorescence ProbesFluorescent ProbesNanoparticle-based ProbesProtease Activity AssaysProtease Activity MappingProtease AssaysProtease ImagingProtein Fluorescence
We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.
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Semiconductor Nanocrystals as Fluorescent Biological Labels
Marcel P. Bruchez, Mario M. Moronne, Peter Gin et al. · Science · 1998 · 8.6K citations