Optimized expression and purification of myristoylated human neuronal calcium sensor 1 in E. coli

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Grzegorz Grynkiewicz, Martin Poenie, Roger Y. Tsien · Journal of Biological Chemistry · 1985 · 21.7K citations · Full text

Natural Sciences, Biochemistry, Chemistry +17

NMRPipe: A multidimensional spectral processing system based on UNIX pipes

Frank Delaglio, Stephan Grzesiek, Geerten W. Vuister et al. · Journal of Biomolecular NMR · 1995 · 16.3K citations

Computing, Computer Science, Computer Systems +14

Protein production by auto-induction in high-density shaking cultures

F. William Studier · Protein Expression and Purification · 2005 · 6.1K citations

Life Sciences, Biochemistry, Biotechnology +12

How to measure and predict the molar absorption coefficient of a protein

C. Nick Pace, F.F. Vajdos, Lanette Fee et al. · Protein Science · 1995 · 3.9K citations · Full text

Life Sciences, Biochemistry, Chemistry +15

Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions

Martial Piotto, Vladimı́r Saudek, Vladimı́r Sklenář · Journal of Biomolecular NMR · 1992 · 3.7K citations

Natural Sciences, Chemistry, Physics +8