Nucleic Acids Research · 1994 · 21 citations · 34 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionPost-translational ModificationProtein TranslationProtein SynthesisRna ModificationRna PolyadenylationTransfer Rna BiochemistryTranslation ElongationTranslational Regulation2'-O-methyl ModificationAlternative Rna ModificationMrna 3' End FormationMrna Translation EfficiencyMrna Translation InitiationTransfer Rna Modifications In Translation
2'-Fluoro- and 2'-amino-2'-deoxynucleoside triphosphates have been used for in vitro transcription of 2'-modified luciferase mRNA. The 2'-modified deoxynucleoside-containing transcripts were tested for the expression of luciferase in X.Laevis oocytes as well as in rabbit reticulocyte lysate. Only 2'-fluoro-2'-deoxy-adenosine-modified mRNA gave rise to luciferase as shown by SDS gel as well as by enzyme activity measurements in vivo as well as in vitro. 2'-Fluoro-2'-deoxy-pyrimidine nucleoside-modified mRNA did not give rise to luciferase activity. However, they directed incorporation of 35S-labeled methionine into peptide fragments in rabbit reticulocyte lysate indicating premature termination of translation. No or only extremely little of such incorporation could be detected with 2'-amino modified transcripts.
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Firefly luciferase gene: structure and expression in mammalian cells.
Jeffrey R. de Wet, Keith V. Wood, M. DeLuca et al. · Molecular and Cellular Biology · 1987 · 3.1K citations · Full text
Firefly Luciferase Gene: Structure and Expression in Mammalian Cells
Jeffrey R. de Wet, Keith V. Wood, Marlene DeLuca et al. · Molecular and Cellular Biology · 1987 · 913 citations