Synthesis of 4‐protio‐3‐carbamoyl‐2,2,5,5‐tetraperdeuteromethyl‐3‐pyrrolin‐1‐yloxy (mHCTPO): A selectively isotopically labeled compound for use in T₂ spin label oxymetry

Abstract

Abstract The title compound (mHCTPO) has been prepared to enhance sensitivity to molecular oxygen concentration in its Electron Spin Resonance (ESR) spectrum. The synthesis involved a novel variant of the traditional nitroxide synthesis leading to a much improved yield of mHCTPO over the several steps of the synthesis. An enhancement of T 2 spin label oxymetry with independent measurment of spin label concentration (from a spectral feature) has been observed. This molecule represents a new class of potentially valuable compounds for in vivo application and for imaging in ESR oxymetry.