Concepedia

Purification of recombinant antigenic epitopes of the human 68-kDa (U1) ribonucleoprotein antigen using the expression system pH6EX3 followed by metal chelating affinity chromatography

Heike Berthold, Mirco Scanarini, Charles C. Abney, Brigitte Frorath, Wolfgang Northemann

Protein Expression and Purification · 1992 · 52 citations · 13 references

DOI

Concepts

References

13

Methods in Enzymology , Vol

S P Colowick, M D Joshi, Jagannathan et al. · 1966 · 26.2K citations

, , +10

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

, , +12

λ ZAP: a bacteriophage λ expression vector with<i>in vivo</i>excision properties

Jay M. Short, Joseph M. Fernandez, Joseph A. Sorge et al. · Nucleic Acids Research · 1988 · 1.4K citations

, , +17

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Erich Hochuli, Heinz Döbeli, Alfred Schacher · Journal of Chromatography A · 1987 · 1.1K citations

, , +12

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

Erich Hochuli, Willi Bannwarth, Heinz Döbeli et al. · Nature Biotechnology · 1988 · 1.1K citations

, , +17