New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
Gene · 1988 · 2.9K citations · 20 references
EngineeringGeneticsSynthetic BiologyMolecular BiologyGenetic EngineeringYeastMolecular GeneticsGene VectorMicrobiologyMolecular MicrobiologyMedicineGenome EditingMicrobial Genetics
20
Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985
15.1K citations
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan · Journal of Molecular Biology · 1983
11.2K citations
Transformation of intact yeast cells treated with alkali cations
Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983
7.7K citations
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985
EngineeringReverse GeneticsEfficient Site-specific Mutagenesis+14
7.3K citations
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Thomas A. Kunkel, John D. Roberts, Richard A. Zakour · Methods in enzymology on CD-ROM/Methods in enzymology · 1987
Efficient Site-specific MutagenesisGeneticsDirected Evolution+5
5.3K citations