A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxyribonucleotide primers

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

Life Sciences, Genetics, Microbiology +12

Polymer support oligonucleotide synthesis XVIII^1.2): use of<i>β</i>-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

N. D. SINHA, J. Biernat, J. F. A. McManus et al. · Nucleic Acids Research · 1984 · 848 citations · Full text

Pharmacy, Biotechnology, Molecular Biology +16

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA

W. John Taylor, Johann Ott, Fritz Eckstein · Nucleic Acids Research · 1985 · 848 citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Improved oligonudeotide site-directed rautagenesis using M13 vectors

Paul Carter, Hugues Bedouelle, Greg Winter · Nucleic Acids Research · 1985 · 527 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +14