Generation of a single-chain Fv fragment for the monitoring of deoxycholic acid residues anchored on endogenous proteins

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

Variant Mrnas, Genetics, Molecular Biology +17

Sequences of proteins of immunological interest

Analytical Biochemistry · 1984 · 3.4K citations

Immunological Interest, Immunogenetics, Medicine +5

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

James S. Huston, Dennis J. Levinson, M Mudgett-Hunter et al. · Proceedings of the National Academy of Sciences · 1988 · 1.6K citations · Full text

E. Coli, Specific Activity, Molecular Biology +18

Single-Chain Antigen-Binding Proteins

Robert E. Bird, Karl D. Hardman, James W. Jacobson et al. · Science · 1988 · 1.5K citations