The Journal of General Physiology · 1935 · 33 citations · 0 references
AgricultureBiochemistryEnzymologyFood ChemistryMilk ChemistryEnzyme KineticsMilk CoagulationMilk EnzymesMilk ProteinsPhysicochemical Properties Of MilkCoagulation MechanismGraduated PipettesMilk Coagulation EnzymesMilk Enzyme KineticsMilk-clotting EnzymesRennet EnzymologyRennet Proteolysis
The rennet activity of chymo-trypsin (or pepsin) is conveniently measured by allowing a standard solution of milk to which chymotrypsin has been added to flow slowly through a graduated pipette and observing the rate and distance of flow of the milk before it clots. The time required for chymo-trypsin to clot milk may be calculated from these observations. The rennet activity is expressed as the reciprocal of the time in minutes required for 1 ml. of enzyme solution to clot 10 ml. of standard milk powder solution.