The Journal of Immunology · 1983 · 30 citations · 0 references
Lymphocyte DevelopmentAdaptive Immune SystemImmunologyImmunologic MechanismIsotype-specific MrnaBcdf GammaLong Non-coding RnaCell SignalingSteady State LevelsT Cell ImmunityGamma-specific Ch3 ProbesGene ExpressionCell BiologyDevelopmental BiologyMurine B CellsImmune Cell DevelopmentGamma-specific MrnaMedicineCell DevelopmentNon-coding Rna
Cytoplasmic RNA was isolated from cells cultured with LPS and LPS plus a T cell-derived supernatant (SN) (PK 7.1) containing B cell differentiation factors. The steady state levels of isotype-specific mRNA were assessed by Northern blot analysis with gamma-specific CH3 probes. It was demonstrated that the SN induces an increase in the level of mRNA for gamma 1 and a concomitant decrease in the levels of mRNA for gamma 2b and gamma 3.