International Journal of Food Sciences and Nutrition · 2015 · 95 citations · 11 references
Life SciencesMicrobiologyNutritionFood MicrobiologyMicrobial MetabolismMicrobial PhysiologyBacterial GrowthLactic Acid BacteriaMicrobial GrowthMicrobial Growth In FoodsPrebioticsProbioticsAgar DilutionBacterial Growth In FoodsBacterial Growth YieldBifidogenic EffectsGrowth In Defined MediaPrebiotic MechanismsProbiotic Microorganisms
XOS has been shown to increase Bifidobacterium counts in vivo without affecting Lactobacillus, and its potential role in obesity management warrants further study. The study evaluated the effect of XOS on the growth of 35 Bifidobacterium and 29 Lactobacillus strains under in vitro conditions. Bacterial strains were identified by 16S rRNA sequencing and their growth was assessed by agar dilution on plates with two‑fold serial dilutions of XOS ranging from 100 to 0.1 mg ml⁻¹. XOS stimulated growth in 86 % of Bifidobacterium strains at 1.56 mg ml⁻¹ and all strains at 6.25 mg ml⁻¹, whereas only 38 % of Lactobacillus strains responded at 1.56 mg ml⁻¹ and 62 % at 6.25 mg ml⁻¹, indicating that XOS preferentially promotes Bifidobacterium growth with limited impact on Lactobacillus.
We recently demonstrated that XOS increased the counts of Bifidobacterium in vivo without increasing Lactobacillus in healthy adults. In the current study, we evaluated the effect of XOS on the growth of 35 Bifidobacterium and 29 Lactobacillus strains in in vitro conditions. Bacteria were identified by 16S rRNA sequence analysis. The growth stimulation was determined by agar dilution technique on plates containing two-fold serial dilutions of XOS (100–0.1 mg/ml). The growth of 86% of Bifidobacterium strains was stimulated at 1.56 mg/ml XOS and 100% at 6.25 mg/ml XOS. The growth of 38% of Lactobacillus strains was stimulated at 1.56 mg/ml XOS and 62% at 6.25 mg/ml XOS; 31% of Lactobacillus were not stimulated by XOS. Our results further suggest that XOS may be beneficial in stimulating intestinal Bifidobacterium without having much effect on Lactobacillus. The potential role for XOS in managing obesity should be investigated further.
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