Quantitative Methods for Anthocyanins.

Abstract

SUMMARY— The method developed consists of extracting the anthocyanins with ethanol‐1.5N hydrochloric acid (85:15) and measuring the O.D. of the extract, diluted with the extracting solvent, at 535 nm. The total anthocyanin content was calculated in absolute quantities with the aid of the extinction coefficients established for the four major cranberry anthocyanins dissolved in the alcoholic solvent system.