The use of homocysteine in the estimation of dehydroascorbic acid

Abstract

Two main methods are in current use for the estimation of dehydroascorbic acid. In the one, developed by Roe and his colleagues (Roe & Kuether, 1943; Roe, Mills, Oesterling & Damron, 1948), the de- hydroascorbic acid is condensed with 2:4-dinitro- phenylhydrazine and the product treated with sulphuric acid to give a red colour, the intensity of which is measured photoelectrically. In a second technique (Tillmans, Hirsch & Siebert, 1932