Microcalorimetric Determination of the Affinity of <i>Saccharomyces cerevisiae</i> for Some Carbohydrate Growth Substrates

Abstract

A microcalorimetric method using a Tian-Calvet apparatus was utilized for measuring the K(m) and V(max) of intact yeast cells for some carbohydrate substrates. These values were obtained for fructose, galactose, and maltose. The heats of ethanolic fermentations were also directly determined, and the results were very close to those obtained by using theoretical calculations and standard values.