PubMed · 1998 · 286 citations · 26 references
Life SciencesCell BiologyOncologyApoptosisCell SignalingSignal TransductionApoptosis In CancerApoptosis RegulationApoptotic SignalingCell DeathDeath ReceptorsProgrammed Cell DeathApoptosis Signaling In CancerApoptosis Signaling PathwaysApoptosis Signaling RegulationCell Death Signaling PathwaysCytokine-induced ApoptosisFas LigandFas Ligand-mediated ApoptosisFas Receptor Signaling
The mechanism by which IFN-gamma inhibits tumor cell growth has not been fully understood. Here we report that IFN-gamma up-regulated the expression of Fas and Fas ligand (FasL) on HT29 cells, a human colon adenocarcinoma cell line, and subsequently induced apoptosis of these cells. The kinetics of cell death in IFN-gamma-treated HT29 cells paralleled the increase in the levels of Fas and FasL expression. We further show that IFN-gamma up-regulated the expression of Fas and FasL in STAT1-transfected U3A cells but not in STAT1-deficient U3A cells. Correspondingly, IFN-gamma induced cell death in STAT1-transfected U3A cells but not in STAT1-deficient U3A cells. IFN-gamma-induced cell death was inhibited by caspase-1 inhibitors. Our results suggest that cell growth inhibition by IFN-gamma is due to apoptosis mediated by Fas and FasL interaction.
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The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis
Naoto Itoh, Shin Yonehara, Ai Ishii et al. · Cell · 1991 · 2.8K citations