MBP Fusion Protein with a Viral Protease Cleavage Site: One-Step Cleavage/Purification of Insoluble Proteins

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Protein Expression, Biochemistry, Single-step Purification +10

Metal chelate affinity chromatography, a new approach to protein fractionation

Jerker Porath, Jan Carlsson, Ingmar Olsson et al. · Nature · 1975 · 2.1K citations

Biochemistry, Natural Sciences, Bioanalysis +8

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Erich Hochuli, Heinz Döbeli, Alfred Schacher · Journal of Chromatography A · 1987 · 1.1K citations

Biochemistry, Natural Sciences, Peptide Engineering +7

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Chu di Guana, Ping Lib, Paul D. Riggsa et al. · Gene · 1988 · 671 citations

Engineering, Protein Expression, Biochemistry +11

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

Claude V. Maina, Paul Riggs, Andres G. Grandea et al. · Gene · 1988 · 540 citations

Maltose-binding Protein, Foreign Proteins, Natural Sciences +8