Journal of Biological Chemistry · 1970 · 40 citations · 9 references
Life SciencesBiochemistryEndocrinologyEnzymologyHormone BiochemistrySteroid BiochemistryHormone MetabolismSteroid BiosynthesisSteroid ConjugationSteroid Hormone BiochemistrySteroid MetabolismSteroidogenic EnzymesAmmonium Sulfate FractionationAmmonium Sulfate PrecipitationEstrogen MetabolismHormone GlucuronidationRat Liver MicrosomesSolubilizationSteroid GlucuronidationSteroid Glucuronosyltransferases
Abstract Steroid glucosyl-, glucuronyl-, and N-acetylglucosaminyltransferases from rabbit liver microsomes have been partially purified by detergent solubilization and ammonium sulfate precipitation. Transfer of glucose to the 17α-hydroxyl group of 17α-estradiol-3-glucuronoside has been shown in the microsomes of kidney and large intestine as well as in those of liver. The liver glucosyltransferase has a specific requirement for UDP-glucose and will transfer glucose from this nucleotide to estrone, 17α-estradiol, and 17β-estradiol. In the case of estrone and 17α-estradiol, it has been established that the transfer is to the phenolic 3-hydroxyl group. The preparation of 17α-estradiol 3-glucoside is reported.
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