Optimization of process parameters for the production of L-asparaginase from an isolated fungus

Abstract

The extracellular L-asparaginase production by fungi isolated from soil samples by using pH and dye based method. Various physical and chemical parameters were optimized under submerged fermentation for Lasparaginase production. Maximum productivity of L-asparaginase (19.5 U/ml) was achieved by employing medium containing 2% (w/v) Lasparagine as substrate concentration, 1%(w/v) glucose as carbon source, 1% (w/v) ammonium sulphate as an additional nitrogen source with the incubation period of 96 h and incubation temperature at 30°C, initial pH 6.5 at an inoculum level 20% (v/v) with 48 h old inoculum was found to be optimum for maximum yield.