An Efficient Synthesis of Enantiomerically Pure Δ‐ and Δ‐Ruthenium(II)‐Labelled Oligonucleotides

Abstract

Abstract The synthesis of a novel tris(bidentate ligand)ruthenium(II) complex 7 and its efficient tethering to the 5′‐end of Oligonucleotides is described. The resulting Δ‐ and Δ‐isomeric ruthenium(II)‐labelled Oligonucleotides 10a‐‐c and 11a‐‐d were separated either by reversed‐phase HPLC or by polyacrylamide gel electrophoresis. The diastereoisomerically pure isomers were fully characterized by UV/VIS and CD spectroscopy, mass spectrometry, and enzymatic digestion with base analysis. We also investigated the thermal denaturation of the hybridized double strands.