Radiocarbon · 1988 · 1.1K citations · 10 references
Life SciencesBiochemistryBiotechnologyBioseparationsProtein BiochemistryExtraction TechniquesProtein AnalysisProtein DegradationProtein PurificationProtein RefoldingProtein SeparationCollagen DegradationCollagen Degradation InhibitionCollagen DenaturationCollagen Matrix DegradationProtein ExtractionPurification
A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
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