ANALYSIS OF FUCOXANTHIN CONTENT AND PURIFICATION OF ALL-TRANS-FUCOXANTHIN FROM<i>Turbinaria turbinata</i>AND<i>Sargassum plagyophyllum</i>BY SiO₂OPEN COLUMN CHROMATOGRAPHY AND REVERSED PHASE-HPLC

Abstract

Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.