Methods in molecular biology · 2013 · 77 citations · 13 references
PharmacyPharmaceutical AnalysisPharmaceuticsBiopharmaceuticsDrug DeliverySpectroscopyAntibody CharacterizationAntibody PharmacokineticsAntibody-antigen RecognitionFluorescence SpectroscopyOptical SpectroscopySpectroscopic Analysis Of PharmaceuticalsAntibody Affinity MeasurementDrug-antibody RatioDrug-to-antibody Ratio OptimizationSpectroscopic Drug Analysis
UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody-drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.
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How to measure and predict the molar absorption coefficient of a protein
C. Nick Pace, F.F. Vajdos, Lanette Fee et al. · Protein Science · 1995 · 3.9K citations · Full text