Whole-slide imaging is a methodological approach within digital pathology that generates high-resolution digital representations of entire histological tissue sections mounted on glass slides. This technique involves systematically acquiring numerous microscopic image tiles or strips across the entire slide surface and computationally stitching them together to form a single, comprehensive digital image. As a fundamental concept and tool in modern pathology research, it enables advanced quantitative analysis, spatial registration across multiple stains or sections, and remote evaluation, significantly advancing diagnostic workflows and the investigation of complex tissue architectures, such as the tumor microenvironment.