Concepedia

Two-dimensional Purification

Two-Dimensional Purification is an enzyme purification strategy that sequentially employs two distinct chromatographic or electrophoretic separation principles to enhance resolution and specificity by exploiting orthogonal physicochemical properties of proteins.

115

Publications

5.2K

Citations

433

Authors

170

Institutions

Publications per year

2017–2026

4

Parents

Publications

115

[11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates

William J. Boyle, Peter van der Geer, Tony Hunter · Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 1.5K citations

, , +14

Investigation into the structural, morphological, mechanical and thermal behaviour of bacterial cellulose after a two-step purification process

Saharman Gea, Christopher T. Reynolds, Nima Roohpour et al. · Bioresource Technology · 2011 · 243 citations

, , +13

Protein Content of Polyhedral Organelles Involved in Coenzyme B <sub>12</sub> -Dependent Degradation of 1,2-Propanediol in <i>Salmonella enterica</i> Serovar Typhimurium LT2

Gregory D. Havemann, Thomas A. Bobik · Journal of Bacteriology · 2003 · 169 citations

, , +17

Chemical Composition of Cholinergic Synaptic Vesicles from Torpedo marmorata Based on Improved Purification

Tomoko Tashiro, Herbert Stadler · European Journal of Biochemistry · 1978 · 120 citations · Full text

, , +17

Partial purification of penicillin acylase from Escherichia coli in poly(ethylene glycol)–sodium citrate aqueous two-phase systems

João Carlos Marcos, Luís P. Fonseca, Maria Teresa Ramalho et al. · Journal of Chromatography B Biomedical Sciences and Applications · 1999 · 113 citations · Full text

, , +12

1–5 of 115

Authors

433

  1. RK
    Rork Kuick

    University of Michigan, United States

    2 publications · 49 citations

    2

    h-index

  2. FT
    Folke Tjerneld

    Lund University, Sweden

    2 publications · 103 citations

    2

    h-index

  3. PR
    Peter R. Jungblut

    Max Planck Institute for Infection Biology, Germany

    2 publications · 65 citations

    2

    h-index

  4. KR
    Kurt Randerath

    Baylor College of Medicine, United States

    2 publications · 84 citations

    2

    h-index

  5. DM
    David M. Lubman

    University of Michigan, United States

    2 publications · 24 citations

    2

    h-index

1–5 of 433

Institutions

170

  1. University of Michigan

    Ann Arbor, United States

    4 h-index

    17 publications

    279 citations

  2. Freie Universität Berlin

    Berlin, Germany

    4 h-index

    7 publications

    187 citations

  3. National Institutes of Health

    Bethesda, United States

    3 h-index

    7 publications

    205 citations

  4. The Ohio State University

    Columbus, United States

    2 h-index

    3 publications

    122 citations

  5. Wayne State University

    Detroit, United States

    2 h-index

    2 publications

    24 citations

  6. National Cancer Institute

    Bethesda, United States

    2 h-index

    5 publications

    175 citations

  7. Radiation Effects Research Foundation

    Nagasaki, Japan

    2 h-index

    5 publications

    110 citations

  8. Macquarie University

    Sydney, Australia

    2 h-index

    4 publications

    136 citations

1–8 of 170

Venues

  1. Journal of Chromatography A

    13 publications · 350 citations

    12

    h-index

  2. Analytical Biochemistry

    8 publications · 255 citations

    8

    h-index

  3. 6

    h-index

  4. Protein Expression and Purification

    5 publications · 133 citations

    5

    h-index

  5. Journal of Chromatography B

    4 publications · 152 citations

    4

    h-index

1–5