Concepedia

Sequence-independent Cloning

Sequence-Independent Cloning is a molecular biology technique that enables the ligation of DNA fragments into a vector without reliance on specific nucleotide sequences at the junction sites, distinguishing it from sequence-dependent cloning methods by facilitating assembly of diverse DNA fragments regardless of their terminal sequences.

23

Publications

2.2K

Citations

105

Authors

52

Institutions

Publications per year

2017–2026

3

Variants

Sequence Independent Cloning

Parents

Publications

23

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

Chaokun Li, Aiyun Wen, Ben-chang Shen et al. · BMC Biotechnology · 2011 · 521 citations · Full text

, , +10

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

Jae-Yeon Jeong, Hyung‐Soon Yim, Ji-Young Ryu et al. · Applied and Environmental Microbiology · 2012 · 469 citations · Full text

, , +14

SLIC: A Method for Sequence- and Ligation-Independent Cloning

Mamie Z. Li, Stephen J. Elledge · Methods in molecular biology · 2012 · 312 citations

, , +11

Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

Milan Blanusa, Alexander Schenk, Hengameh Sadeghi et al. · Analytical Biochemistry · 2010 · 117 citations

, , +13

A general method for inserting specific DNA sequences into cloning vehicles

Chander P. Bahl, Kenneth J. Marians, Ray Wü et al. · Gene · 1976 · 112 citations

, , +13

1–5 of 23

Authors

105

  1. GL
    G.M.A. Lima

    MAX IV Laboratory, Sweden

    1 publications · 11 citations

    1

    h-index

  2. PC
    Peng Chen

    Chinese Academy of Sciences, China

    1 publications · 24 citations

    1

    h-index

  3. ML
    María Lluch‐Senar

    Centre for Genomic Regulation, Spain

    1 publications · 13 citations

    1

    h-index

  4. AG
    Anton Glieder

    Graz University of Technology, Austria

    1 publications · 28 citations

    1

    h-index

  5. SH
    S. Holloway

    University of Liverpool, United Kingdom

    1 publications · 43 citations

    1

    h-index

1–5 of 105

Institutions

52

  1. Universidade de São Paulo

    São Paulo, Brazil

    2 h-index

    8 publications

    112 citations

  2. Centre for Genomic Regulation

    Barcelona, Spain

    1 h-index

    5 publications

    65 citations

  3. Board of the Swiss Federal Institutes of Technology

    Zurich, Switzerland

    1 h-index

    3 publications

    87 citations

  4. Imperial College London

    London, United Kingdom

    1 h-index

    4 publications

    148 citations

  5. University of Manchester

    Manchester, United Kingdom

    1 h-index

    5 publications

    215 citations

  6. University of Basel

    Basel, Switzerland

    1 h-index

    3 publications

    87 citations

  7. Tsinghua University

    Beijing, China

    1 h-index

    4 publications

    124 citations

  8. Reef Ecologic

    Townsville, Australia

    1 h-index

    1 publications

    31 citations

1–8 of 52

Venues

  1. Gene

    3 publications · 213 citations

    3

    h-index

  2. Analytical Biochemistry

    2 publications · 136 citations

    2

    h-index

  3. PLoS ONE

    2 publications · 108 citations

    2

    h-index

  4. Nucleic Acids Research

    2 publications · 50 citations

    2

    h-index

  5. Methods in molecular biology

    Book series · 1 publications · 312 citations

    1

    h-index

1–5