Concepedia

Multi-site Mutagenesis

Multi-Site Mutagenesis is a molecular biology technique that enables the simultaneous introduction of multiple targeted mutations at distinct sites within a DNA sequence through a single engineered construct, distinguishing it from single-site approaches by its capacity for parallel genetic modifications.

83

Publications

7.4K

Citations

359

Authors

148

Institutions

Publications per year

2017–2026

17

Parents

Publications

83

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Huanting Liu, James H. Naismith · BMC Biotechnology · 2008 · 1.3K citations · Full text

, , +13

A general method for site-directed mutagenesis in prokaryotes

Gary Ruvkun, Frederick M. Ausubel · Nature · 1981 · 698 citations

, , +13

Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange <sup>TM</sup> Site-Directed Mutagenesis

Wenyan Wang, Bruce A. Malcolm · BioTechniques · 1999 · 602 citations · Full text

, , +15

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

Chao Li, Rui Zhang, Xiangbing Meng et al. · Nature Biotechnology · 2020 · 424 citations

, , +17

Suggestions for “safe” residue substitutions in site-directed mutagenesis

Domenico Bordo, Patrick Argos · Journal of Molecular Biology · 1991 · 384 citations

, , +9

1–5 of 83

Authors

359

  1. US
    Ulrich Schwaneberg

    RWTH Aachen University, Germany

    3 publications · 155 citations

    3

    h-index

  2. AD
    Alexander Dennig

    RWTH Aachen University, Germany

    2 publications · 137 citations

    2

    h-index

  3. BA
    Bruce A. Malcolm

    University of Alberta, Canada

    2 publications · 736 citations

    2

    h-index

  4. AV
    Amol V. Shivange

    RWTH Aachen University, Germany

    2 publications · 137 citations

    2

    h-index

  5. DO
    Dieter Oesterhelt

    Max Planck Institute of Biochemistry, Germany

    1 publications · 43 citations

    1

    h-index

1–5 of 359

Institutions

148

  1. Chinese Academy of Sciences

    Beijing, China

    4 h-index

    14 publications

    3.7K citations

  2. RWTH Aachen University

    Aachen, Germany

    4 h-index

    16 publications

    664 citations

  3. Washington University in St. Louis

    St Louis, United States

    2 h-index

    10 publications

    155 citations

  4. University of Auckland

    Auckland, New Zealand

    2 h-index

    2 publications

    41 citations

  5. Nankai University

    Tianjin, China

    2 h-index

    5 publications

    174 citations

  6. Shenzhen University

    Shenzhen, China

    2 h-index

    6 publications

    98 citations

  7. Stanford University

    Stanford, United States

    2 h-index

    21 publications

    1.8K citations

  8. Centre National de la Recherche Scientifique

    Paris, France

    2 h-index

    5 publications

    651 citations

1–8 of 148

Venues

  1. Analytical Biochemistry

    11 publications · 344 citations

    10

    h-index

  2. Nucleic Acids Research

    6 publications · 869 citations

    6

    h-index

  3. BioTechniques

    5 publications · 705 citations

    5

    h-index

  4. Applied Microbiology and Biotechnology

    4 publications · 248 citations

    4

    h-index

  5. Gene

    4 publications · 419 citations

    4

    h-index

1–5