Concepedia

Multi-fragment Assembly

Multi-Fragment Assembly is a molecular cloning method that enables the simultaneous and directional joining of three or more DNA fragments into a single contiguous molecule through sequence-specific overlaps or enzymatic assembly strategies.

64

Publications

5.3K

Citations

279

Authors

105

Institutions

Publications per year

2017–2026

15

Parents

Publications

64

Enzymatic Assembly of Overlapping DNA Fragments

Daniel G. Gibson · Methods in enzymology on CD-ROM/Methods in enzymology · 2011 · 796 citations · Full text

, , +12

Folding non-homologous proteins by coupling deep-learning contact maps with I-TASSER assembly simulations

Wei Zheng, Chengxin Zhang, Yang Li et al. · Cell Reports Methods · 2021 · 585 citations · Full text

, , +13

Short read fragment assembly of bacterial genomes

Mark Chaisson, Pavel A. Pevzner · Genome Research · 2007 · 446 citations · Full text

, , +17

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis

Yongzhen Xia, Kai Li, Jingjing Li et al. · Nucleic Acids Research · 2018 · 350 citations · Full text

, , +15

Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously

Nikolai A Shevchuk · Nucleic Acids Research · 2004 · 341 citations · Full text

, , +16

1–5 of 64

Authors

279

  1. AB
    Amos B. Smith

    University of Pennsylvania, United States

    2 publications · 126 citations

    2

    h-index

  2. GC
    Guo‐Qiang Chen

    Tsinghua University, China

    2 publications · 48 citations

    2

    h-index

  3. LZ
    Linghang Zhuang

    Monell Chemical Senses Center, United States

    2 publications · 126 citations

    2

    h-index

  4. XW
    Xia Wu

    Central China Normal University, China

    2 publications · 122 citations

    2

    h-index

  5. ZS
    Zhenyu Shi

    Henan University, China

    2 publications · 48 citations

    2

    h-index

1–5 of 279

Institutions

105

  1. University of Michigan

    Ann Arbor, United States

    2 h-index

    8 publications

    3.7K citations

  2. Tsinghua University

    Beijing, China

    2 h-index

    9 publications

    209 citations

  3. Harvard University

    Cambridge, United States

    2 h-index

    4 publications

    204 citations

  4. Monell Chemical Senses Center

    Philadelphia, United States

    2 h-index

    18 publications

    1.1K citations

  5. University of California San Diego

    San Diego, United States

    2 h-index

    4 publications

    1.4K citations

  6. University of Pennsylvania

    Philadelphia, United States

    2 h-index

    18 publications

    1.1K citations

  7. Science for Life Laboratory

    Stockholm, Sweden

    2 h-index

    3 publications

    73 citations

  8. Center for Life Sciences

    Beijing, China

    2 h-index

    9 publications

    209 citations

1–8 of 105

Venues

  1. Nucleic Acids Research

    7 publications · 891 citations

    7

    h-index

  2. Scientific Reports

    7 publications · 462 citations

    7

    h-index

  3. PLoS ONE

    5 publications · 304 citations

    5

    h-index

  4. Organic Letters

    OL · 3 publications · 165 citations

    3

    h-index

  5. Angewandte Chemie International Edition

    3 publications · 214 citations

    3

    h-index

1–5