Concepedia

Glycosynthase Engineering

Glycosynthase Engineering is a protein engineering strategy that involves the rational design or directed evolution of glycosidases to create mutant enzymes capable of catalyzing glycosidic bond formation without hydrolytic activity, thereby enabling efficient and stereoselective biocatalytic glycosylation.

57

Publications

2.9K

Citations

195

Authors

78

Institutions

Publications per year

2017–2026

9

Parents

Publications

57

Glycosynthases:  Mutant Glycosidases for Oligosaccharide Synthesis

Lloyd Mackenzie, Qingping Wang, R. Antony J. Warren et al. · Journal of the American Chemical Society · 1998 · 535 citations

, , +15

Efficient Glycosynthase Mutant Derived from Mucor hiemalis Endo-β-N-acetylglucosaminidase Capable of Transferring Oligosaccharide from Both Sugar Oxazoline and Natural N-Glycan

Midori Umekawa, Cishan Li, Takayuki Higashiyama et al. · Journal of Biological Chemistry · 2009 · 150 citations · Full text

, , +16

Directed Evolution of a Glycosynthase from Agrobacterium sp. Increases Its Catalytic Activity Dramatically and Expands Its Substrate Repertoire

Young‐Wan Kim, Seung Seo Lee, R. Antony J. Warren et al. · Journal of Biological Chemistry · 2004 · 125 citations · Full text

, , +17

The E358S mutant of <i>Agrobacterium</i> sp. β‐glucosidase is a greatly improved glycosynthase

Christoph Mayer, David L. Zechel, Stephen P. Reid et al. · FEBS Letters · 2000 · 121 citations

, , +17

1,2‐α‐<scp>l</scp>‐Fucosynthase: A glycosynthase derived from an inverting α‐glycosidase with an unusual reaction mechanism

Jun Wada, Yuji Honda, Masamichi Nagae et al. · FEBS Letters · 2008 · 106 citations

, , +17

1–5 of 57

Authors

195

  1. SG
    Stephen G. Withers

    University of British Columbia, Canada

    13 publications · 1.3K citations

    13

    h-index

  2. RA
    R. Antony J. Warren

    University of British Columbia, Canada

    7 publications · 1.1K citations

    7

    h-index

  3. MM
    Marco Moracci

    Institute of Protein Biochemistry, Italy

    7 publications · 332 citations

    7

    h-index

  4. MR
    Mosé Rossi

    Institute of Protein Biochemistry, Italy

    6 publications · 259 citations

    6

    h-index

  5. AP
    Antoni Planas

    Institut Químic de Sarrià, Spain

    6 publications · 165 citations

    6

    h-index

1–5 of 195

Institutions

78

  1. University of British Columbia

    Vancouver, Canada

    12 h-index

    51 publications

    5.6K citations

  2. Centre National de la Recherche Scientifique

    Paris, France

    6 h-index

    27 publications

    816 citations

  3. Institute of Protein Biochemistry

    Naples, Italy

    6 h-index

    22 publications

    1.1K citations

  4. Institut Químic de Sarrià

    Barcelona, Spain

    6 h-index

    17 publications

    478 citations

  5. Universitat Ramon Llull

    Barcelona, Spain

    6 h-index

    17 publications

    478 citations

  6. National Research Council

    Rome, Italy

    5 h-index

    22 publications

    986 citations

  7. Centre de Recherches sur les Macromolécules Végétales

    Gières, France

    3 h-index

    12 publications

    561 citations

  8. Kyoto University

    Kyoto, Japan

    3 h-index

    7 publications

    828 citations

1–8 of 78

Venues

  1. Chemistry & Biology

    3 publications · 220 citations

    3

    h-index

  2. Glycobiology

    3 publications · 98 citations

    3

    h-index

  3. Journal of the American Chemical Society

    JACS · 3 publications · 680 citations

    3

    h-index

  4. Biochemical Journal

    BJ · 2 publications · 67 citations

    2

    h-index

  5. Carbohydrate Research

    2 publications · 35 citations

    2

    h-index

1–5