Concepedia

Dna Polymerase Engineering

DNA Polymerase Engineering is a methodological framework within protein engineering that involves the rational design or directed evolution of DNA polymerases to alter their fidelity, substrate specificity, or catalytic properties for applications in error-prone mutagenesis.

169

Publications

32.5K

Citations

674

Authors

236

Institutions

Publications per year

2017–2026

42

Parents

Publications

169

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations

, , +12

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

RK Saiki, DH Gelfand, Susanne Stoffel et al. · Science · 1988 · 4.3K citations

, , +12

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

Wayne M. Barnes · Proceedings of the National Academy of Sciences · 1994 · 1K citations · Full text

, , +15

An attempt to unify the structure of polymerases

Marc Delarue, Olivier Poch, Noël Tordo et al. · Protein Engineering Design and Selection · 1990 · 637 citations

, , +15

High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus

Kelly S. Lundberg, Dan D. Shoemaker, Michael W. W. Adams et al. · Gene · 1991 · 574 citations

, , +8

1–5 of 169

Authors

674

  1. JC
    John C. Chaput

    University of California, Irvine, United States

    11 publications · 761 citations

    11

    h-index

  2. PH
    Philipp Holliger

    MRC Laboratory of Molecular Biology, United Kingdom

    6 publications · 467 citations

    6

    h-index

  3. MR
    Matthew R. Dunn

    University of California, Irvine, United States

    6 publications · 406 citations

    6

    h-index

  4. MK
    Masayasu Kuwahara

    Gunma University, Japan

    5 publications · 158 citations

    5

    h-index

  5. MH
    Michal Hocek

    Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Czechia

    5 publications · 233 citations

    5

    h-index

1–5 of 674

Institutions

236

  1. University of California, Irvine

    Irvine, United States

    9 h-index

    26 publications

    1.6K citations

  2. Harvard University

    Cambridge, United States

    8 h-index

    33 publications

    2.2K citations

  3. Centre National de la Recherche Scientifique

    Paris, France

    7 h-index

    27 publications

    1.4K citations

  4. MRC Laboratory of Molecular Biology

    Cambridge, United Kingdom

    6 h-index

    19 publications

    1.6K citations

  5. Gunma University

    Maebashi, Japan

    5 h-index

    25 publications

    772 citations

  6. The University of Tokyo

    Tokyo, Japan

    5 h-index

    24 publications

    2K citations

  7. Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

    Prague, Czechia

    5 h-index

    15 publications

    807 citations

  8. Scripps Research Institute

    San Diego, United States

    5 h-index

    20 publications

    1.8K citations

1–8 of 236

Venues

  1. Nucleic Acids Research

    16 publications · 1.1K citations

    14

    h-index

  2. Journal of the American Chemical Society

    JACS · 10 publications · 909 citations

    10

    h-index

  3. Proceedings of the National Academy of Sciences

    PNAS · 8 publications · 1.9K citations

    8

    h-index

  4. Gene

    8 publications · 979 citations

    8

    h-index

  5. ACS Synthetic Biology

    7 publications · 308 citations

    7

    h-index

1–5