Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos

Erwei Zuo, Yidi Sun, Wei Wu, +7

Science

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice

Haibo Zhou, Jinlin Su, Xinde Hu, +19

Cell

Neurodegenerative DiseasesNeuroregenerationMedicineGene EditingNeurology +10

Adaptation and possible ancient interspecies introgression in pigs identified by whole-genome sequencing

Huashui Ai, Xiaodong Fang, Bin Yang, +23

Nature Genetics

BiologyWhole-genome SequencingComparative GenomicsGeneticsEvolutionary Biology +9

Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes

Chunlong Xu, Yingsi Zhou, Qingquan Xiao, +13

Nature Methods

EngineeringProgrammable RnaRna BiologyGenetic EngineeringMolecular Biology +7

In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Haibo Zhou, Junlai Liu, Changyang Zhou, +19

Nature Neuroscience

Transcriptional RegulationSystems BiologyIn Vivo Gene TherapyMultiple GenesCrispr–dcas9-activator Transgenic Mice +10

Expanded catalog of microbial genes and metagenome-assembled genomes from the pig gut microbiome

Congying Chen, Yunyan Zhou, Hao Fu, +7

Nature Communications

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects

Huawei Tong, Jia Huang, Qingquan Xiao, +20

Nature Biotechnology

Targeted Rna DegradationRna ProcessingSystems BiologyEngineeringOff-target Effect +8

ABO genotype alters the gut microbiota by regulating GalNAc levels in pigs

Hui Yang, Jinyuan Wu, Xiaochang Huang, +26

Nature

BiologyMicrobiota StructureMicrobiota FunctionDysbiosisHost-microbe Interactions +11

A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects

Erwei Zuo, Yidi Sun, Tanglong Yuan, +8

Nature Methods

EngineeringGenome SurgeryNatural SciencesHigh On-target ActivitySynthetic Biology +10

Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice

Sikai Ling, Shiqi Yang, Xinde Hu, +10

Nature Biomedical Engineering

Ocular DiseaseVitreous BodyLentiviral DeliveryOphthalmologyCo-packaged Cas9 Mrna +4