Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3

Atsushi Miyajima, Jolanda Schreurs, Keiko Otsu, +3

Gene

InflammationCytokineAllergyInsect Baculovirus VectorBombyx Mori +7

Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi

Wanderson D. DaRocha, Keiko Otsu, Santuza Maria Ribeiro Teixeira, +1

Molecular and Biochemical Parasitology

Trypanosoma CruziAfrican TrypanosomiasisNatural SciencesGeneticsRna Biology +6

Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones.

T Otsuka, Atsushi Miyajima, Neal Brown, +7

The Journal of Immunology

InflammationCytokineT Cell ClonesAdaptive Immune SystemImmunology +13

Expression of murine and human granulocyte-macrophage colony-stimulating factors in S. cerevisiae: mutagenesis of the potential glycosylation sites.

Atsushi Miyajima, Keiko Otsu, Jolanda Schreurs, +3

The EMBO Journal

Fungal Cell BiologyGranulocyteCell SignalingNatural SciencesPathogenesis +12

Phenotypic changes of bone marrow-derived mast cells after intraperitoneal transfer into W/Wv mice that are genetically deficient in mast cells.

Keiko Otsu, Toru Nakano, Yuzuru Kanakura, +6

The Journal of Experimental Medicine

Tissue EngineeringCell TherapySclerostinW/wv MiceMast Cell Disorder +16

Trypanosoma cruzi expresses diverse repetitive protein antigens

Daniel F. Hoft, K S Kim, Keiko Otsu, +5

Infection and Immunity

Trypanosoma CruziParasitic DiseaseCorresponding MrnasAfrican TrypanosomiasisMedicine +12

Purification and analysis of the core protein of the protease-resistant intracellular chondroitin sulfate E proteoglycan from the interleukin 3-dependent mouse mast cell.

R L Stevens, Keiko Otsu, K. Frank Austen

Journal of Biological Chemistry

SclerostinMast Cell DisorderImmunologyGlycobiologyCore Protein +20

Secretion of mature mouse interleukin-2 by Saccharomyces cerevisiae: use of a general secretion vector containing promoter and leader sequences of the mating pheromone α-factor

Atsushi Miyajima, M W Bond, Keiko Otsu, +2

Gene

CytokineSaccharomyces CerevisiaeImmune Cell DevelopmentPathogenesisImmunology +7

A Function for a Specific Zinc Metalloprotease of African Trypanosomes

Paul M. Grandgenett, Keiko Otsu, Helen Rose Wilson, +2

PLoS Pathogens

Recombinant Leishmania Hsp90 and Hsp70 are recognized by sera from visceral leishmaniasis patients but not Chagas' disease patients

C.R. Andrade, Louis V. Kirchhoff, J E Donelson, +1

Journal of Clinical Microbiology

ImmunodeficienciesImmunologyPathologyAntigen ProcessingRecombinant Leishmania Hsp90 +17