Mild Method for Succinimide Hydrolysis on ADCs: Impact on ADC Potency, Stability, Exposure, and Efficacy

L. Nathan Tumey, Manoj B. Charati, Tao He, +9

Bioconjugate Chemistry

Medicinal ChemistryMaleimide-based AdcsBiochemistryAdc PotencyMedicine +12

Differential Proteomic Analysis of Bronchoalveolar Lavage Fluid in Asthmatics following Segmental Antigen Challenge

Jiang Wu, Michiko Kobayashi, Eric Sousa, +8

Molecular & Cellular Proteomics

Acid-Labile Isotope-Coded Extractants:  A Class of Reagents for Quantitative Mass Spectrometric Analysis of Complex Protein Mixtures

Yongchang Qiu, Eric Sousa, Rodney M. Hewick, +1

Analytical Chemistry

Peptide MixturesBiological Mass SpectrometryProtein AnalysisMolecular BiologyProtein Purification +22

Integrating titania enrichment, iTRAQ labeling, and Orbitrap CID‐HCD for global identification and quantitative analysis of phosphopeptides

Jiang Wu, Peter Warren, Quazi Shakey, +4

PROTEOMICS

Proteomic Identification of Endothelial Proteins Isolated in Situ from Atherosclerotic Aorta via Systemic Perfusion

Jiang Wu, Wei Liu, Eric Sousa, +9

Journal of Proteome Research

Vascular DiseaseImmunologySystemic PerfusionInflammationProteomic Technology +15

Glyco-engineered Long Acting FGF21 Variant with Optimal Pharmaceutical and Pharmacokinetic Properties to Enable Weekly to Twice Monthly Subcutaneous Dosing

Yan Weng, Tetsuya Ishino, Annette Sievers, +23

Scientific Reports

Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease

Xiaotian Zhong, Srinath Jagarlapudi, Yan Weng, +28

Journal of Biological Chemistry

Pyroglutamate and O-Linked Glycan Determine Functional Production of Anti-IL17A and Anti-IL22 Peptide-Antibody Bispecific Genetic Fusions

Xiaotian Zhong, Elizabeth Kieras, Eric Sousa, +12

Journal of Biological Chemistry

ImmunologyImmunologic MechanismAntigen ProcessingImmunotherapeuticsImmunotherapy +14

Mechanistic understanding of the cysteine capping modifications of antibodies enables selective chemical engineering in live mammalian cells

Xiaotian Zhong, Tao He, Amar S. Prashad, +16

Journal of Biotechnology

Immunocytochemical TechniqueSignal TransductionBiochemistryImmunotherapyMechanistic Understanding +12

Primary sequence determination of a monoclonal antibody against α-synuclein using a novel mass spectrometry-based approach

Eric Sousa, Stéphane Olland, Heather H. Shih, +6

International Journal of Mass Spectrometry

MedicineNatural SciencesBioanalysisMass SpectrometryImmunology +9