Journal of Biological Chemistry · 1933 · 15 citations · 6 references
MedicineNutritionNutritional SciencesHepatologyLyophilizationMetabolismHepatic MetabolismLiver DiseaseLiver PhysiologyMetabolic Liver DiseaseNutrition In Liver DiseaseHepatic Lipid Metabolism
MethodsLiver-The main portion of the liver was transferred to a clock glass and cut fine with scissors and then placed in a vacuum drying oven at a temperature of 48'.After about 20 hours, the tissue was ground in a mortar and then returned to the oven until it was completely dry.It was then transferred to a 100 cc.volumetric flask and about 75 cc. of 95 per cent alcohol were added.If the weight of the fresh liver was more than 5 gm., twice as much of 93
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THE COLORIMETRIC DETERMINATION OF PHOSPHORUS
Cyrus H. Fiske, Y. Subbarow · Journal of Biological Chemistry · 1925 · 19.1K citations · Full text