The unusual stability of the IS10 anti‐sense RNA is critical for its function and is determined by the structure of its stem‐domain.

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Improved single and multicopy lac-based cloning vectors for protein and operon fusions

Robert W. Simons, Fariba Houman, Nancy Kleckner · Gene · 1987 · 1.5K citations

Life Sciences, Biotechnology, Molecular Biology +13

Improved free-energy parameters for predictions of RNA duplex stability.

Susan M. Freier, Ryszard Kierzek, J. A. Jaeger et al. · Proceedings of the National Academy of Sciences · 1986 · 1.5K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +17

New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition

Jeffrey C. Way, Michael A. Davis, Donald Morisato et al. · Gene · 1984 · 609 citations

Life Sciences, Genetics, Molecular Genetics +12

Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.

William P. Donovan, Sidney R. Kushner · Proceedings of the National Academy of Sciences · 1986 · 430 citations

Life Sciences, Microbiology, Molecular Biology +14