Immobilon-P transfer membrane: applications and utility in protein biochemical analysis.

Abstract

Polyvinylidene fluoride membrane for protein-blotting applications was introduced by Millipore Corporation in 1986. The membrane, designated variously as Immobilon-PVDF, Immobilon-P Transfer Membrane and Immobilon-P, has been widely used in protein biochemical analysis. Applications developed using Millipore's polyvinylidene fluoride membrane span immunochemistry, cell biology and protein structural characterization. Additionally, protein analysis on Immobilon-P membrane has been interfaced to a range of separation methodologies including high-pressure liquid chromatography and both one- and two-dimensional electrophoresis.