Paroxysmal Nocturnal Hemoglobinuria

Abstract

Abstract 1. Crude bovine thrombin contains most, if not all, the proteins of bovine serum. 2. The effect of crude bovine thrombin in enhancing the lysis of paroxysmal nocturnal hemoglobinuria erythrocytes has been shown to be an effect on the erythrocytes themselves. 3. By enzyme inactivation, absorption technics and Sephadex chromatography the thrombic clotting activity has been separated from the PNH enhancing activity nullifying any role of thrombic clotting activity in the enhancement of PNH hemolysis by crude bovine thrombin.