Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis

Abstract

Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na + importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na + importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na + accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na + uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO 3 − -promoted Na + , K + , and Li + importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na + import and preventing excessive Na + accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na + transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation.