Integration of multi-omics data accelerates molecular analysis of common wheat traits

Abstract

Integration of multi-omics data can provide information on biomolecules from different layers to illustrate the complex biology systematically. Here, we build a multi-omics atlas containing 132,570 transcripts, 44,473 proteins, 19,970 phosphoproteins, and 12,427 acetylproteins across wheat vegetative and reproductive phases. Using this atlas, we elucidate transcriptional regulation network, contributions of post-translational modification (PTM) and transcript level to protein abundance, and biased homoeolog expression and PTM in wheat. The genes/proteins related to wheat development and disease resistance are systematically analyzed, thus identifying phosphorylation and/or acetylation modifications for the seed proteins controlling wheat grain quality and the disease resistance-related genes. Lastly, a unique protein module TaHDA9-TaP5CS1, specifying de-acetylation of TaP5CS1 by TaHDA9, is discovered, which regulates wheat resistance to Fusarium crown rot via increasing proline content. Our atlas holds great promise for fast-tracking molecular biology and breeding studies in wheat and related crops. Limited high quality multi-omics resources are available for plants with complex genomes. Here, authors generate transcriptome, proteome, phosphoproteome, and acetylproteome data sets across wheat developmental stages and reveal de-acetylation of TaP5CS1 by TaHDA9 regulates Fusarium crown rot resistance.