Lab on a Chip · 2023 · 22 citations · 20 references
Life SciencesBiotechnologyMolecular BiologyBiomemsNucleic Acid BiochemistryNucleic Acid ChemistryMicrofluidic BiosensorsMicrofluidicsPolymerase Chain ReactionDigital Molecular DetectionDigital PcrNucleic Acid DetectionPolymerase Chain Reaction (Pcr) AssaysQuantitative Pcr DetectionReal-time Polymerase Chain Reaction (Pcr)
heating on the chip were well characterized. Finally, the performance of the platform was validated through the analysis of the EGFR L858R mutation in lung cancer. The proposed three-in-one ddPCR platform shows great practicability in ultrasensitive nucleic acid testing. By virtue of its sensitivity, practicability, and cost-effectiveness, the ddPCR can serve as a universal detection platform for monitoring nucleic acid in the fields of tumor diagnosis, pathogen detection, and prenatal diagnosis.
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Bert Vogelstein, Kenneth W. Kinzler · Proceedings of the National Academy of Sciences · 1999 · 1.7K citations