Solar-powered multi-organism symbiont mimic system for beyond natural synthesis of polypeptides from CO ₂ and N ₂

Abstract

Developing artificial symbionts beyond natural synthesis limitations would bring revolutionary contributions to agriculture, medicine, environment, etc. Here, we initiated a solar-driven multi-organism symbiont, which was assembled by the CO 2 fixation module of Synechocystis sp., N 2 fixation module of Rhodopseudomonas palustris , biofunctional polypeptides synthesis module of Bacillus licheniformis , and the electron transfer module of conductive cationic poly(fluorene- co -phenylene) derivative. The modular design broke the pathway to synthesize γ-polyglutamic acid (γ-PGA) using CO 2 and N 2 , attributing to the artificially constructed direct interspecific substance and electron transfer. So, the intracellular ATP and NADPH were enhanced by 69 and 30%, respectively, and the produced γ-PGA was enhanced by 104%. The strategy was further extended to produce a commercial antibiotic of bacitracin A. These achievements improve the selectivity and yield of functional polypeptides with one click by CO 2 and N 2 , and also provide an innovative strategy for creating photosynthetic systems on demand.