Gut microbiota profiling in aged dogs after feeding pet food contained Hericium erinaceus

Abstract

Health concern of dogs is the most important issue for pet owners. People who have companied the dogs long-term provide the utmost cares for their well-being and healthy life. Recently, it was revealed that the population and types of gut microbiota affect the metabolism and immunity of the host. However, there is little information on the gut microbiome of dogs. <i>Hericium erinaceus</i> (<i>H. erinaceus</i>; HE) is one of the well-known medicinal mushrooms and has multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine, hericerin, erinacines, etc. Here we tested a pet food that contained <i>H. erinaceus</i> for improvement in the gut microbiota environment of aged dogs. A total of 18 dogs, each 11 years old, were utilized. For sixteen weeks, the dogs were fed with 0.4 g of H. erinaceus (HE-L), or 0.8 g (HE-H), or without <i>H. erinaceus</i> (CON) per body weight (kg) with daily diets (n = 6 per group). Taxonomic analysis was performed using metagenomics to investigate the difference in the gut microbiome. Resulting from principal coordinates analysis (PCoA) to confirm the distance difference between the groups, there was a significant difference between HE-H and CON due to weighted Unique fraction metric (Unifrac) distance (<i>p</i> = 0.047), but HE-L did not have a statistical difference compared to that of CON. Additionally, the result of Linear discriminate analysis of effect size (LEfSe) showed that phylum <i>Bacteroidetes</i> in HE-H and its order Bacteroidales increased, compared to that of CON, Additionally, phylum <i>Firmicutes</i> in HE-H, and its genera (<i>Streptococcus</i>, <i>Tyzzerella</i>) were reduced. Furthermore, at the family level, <i>Campylobacteraceae</i> and its genus <i>Campylobacter</i> in HE-H was decreased compared to that of CON. Summarily, our data demonstrated that the intake of <i>H. erinaceus</i> can regulate the gut microbial community in aged dogs, and an adequate supply of HE on pet diets would possibly improve immunity and anti-obesity on gut-microbiota in dogs.