Clonal Propagation of Coffea arabica L. from Callus Culture1

Abstract

Abstract Large numbers of coffee organoids were produced from callus cultures derived from leaf blade segments. Organoids developed from callus grown on a modified Linsmaier-Skoog medium, containing 0.1 mg per liter kinetin and 0.1 mg per liter 2,4-dichlorophenoxyacetic acid (2,4-D), typically consisted of rudimentary stems and leaves only. Roots were formed and the organoids developed into coffee plants upon transfer of the organoids to a Gresshoff-Doy medium containing 0.1 mg per liter naphthaleneacetic acid (NAA) but no kinetin. The plants continued to grow after being transferred to soil.