Concepedia

Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

Kun Zhang, Xingpeng Duan, Peng Cai, Linhui Gao, Xiaoyan Wu, Lun Yao, Yongjin J. Zhou

Microbial Cell Factories · 2022 · 21 citations · 21 references

DOIFull text

Open access

Abstract

Fusion expression of the endogenous exonuclease Mre11 with Cas9 enhances homologous recombination efficiency in P. pastoris. The strategy described here should facilitate the metabolic engineering of P. pastoris toward high-level production of value-added compounds.

References

21

DNA helicase Srs2 disrupts the Rad51 presynaptic filament

Lumír Krejčí, Stephen Van Komen, Ying Li et al. · Nature · 2003 · 629 citations

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Extraction of genomic DNA from yeasts for PCR-based applications

Marko Lõoke, Kersti Kristjuhan, Arnold Kristjuhan · BioTechniques · 2011 · 582 citations · Full text

Condensed Protocol for Competent Cell Preparation and Transformation of the Methylotrophic Yeast Pichia Pastoris

Joan Lin‐Cereghino, William W. Wong, See Xiong et al. · BioTechniques · 2005 · 376 citations · Full text

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Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

Laura Näätsaari, Beate Mistlberger, Claudia Rüth et al. · PLoS ONE · 2012 · 292 citations · Full text

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Barriers and opportunities in bio-based production of hydrocarbons

Yongjin J. Zhou, Eduard J. Kerkhoven, Jens Nielsen · Nature Energy · 2018 · 208 citations

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