Simple synthesis of soft, tough, and cytocompatible biohybrid composites

Abstract

Collagen is the most abundant component of mammalian extracellular matrices. As such, the development of materials that mimic the biological and mechanical properties of collagenous tissues is an enduring goal of the biomaterials community. Despite the development of molded and 3D printed collagen hydrogel platforms, their use as biomaterials and tissue engineering scaffolds is hindered by either low stiffness and toughness or processing complexity. Here, we demonstrate the development of stiff and tough biohybrid composites by combining collagen with a zwitterionic hydrogel through simple mixing. This combination led to the self-assembly of a nanostructured fibrillar network of collagen that was ionically linked to the surrounding zwitterionic hydrogel matrix, leading to a composite microstructure reminiscent of soft biological tissues. The addition of 5–15 mg mL −1 collagen and the formation of nanostructured fibrils increased the elastic modulus of the composite system by 40% compared to the base zwitterionic matrix. Most notably, the addition of collagen increased the fracture energy nearly 11-fold ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"> <mml:mrow> <mml:mtext>Γ</mml:mtext> <mml:mo>=</mml:mo> </mml:mrow> </mml:math> 180 J m −2 ) and clearly delayed crack initiation and propagation. These composites exhibit elastic modulus ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"> <mml:mrow> <mml:mi>E</mml:mi> <mml:mo>=</mml:mo> </mml:mrow> </mml:math> 0.180 MJ) and toughness ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"> <mml:mrow> <mml:msup> <mml:mrow> <mml:mi>W</mml:mi> </mml:mrow> <mml:mo>*</mml:mo> </mml:msup> <mml:mo>=</mml:mo> <mml:mo> </mml:mo> </mml:mrow> </mml:math> 0.617 MJ m −3 ) approaching that of biological tissues such as articular cartilage. Maintenance of the fibrillar structure of collagen also greatly enhanced cytocompatibility, improving cell adhesion more than 100-fold with &gt;90% cell viability.