Journal of the American Society of Brewing Chemists · 1992 · 10 citations · 0 references
Life SciencesBiotechnologyGeneticsGene ExpressionGenetic EngineeringMicrobial GeneticsAlcoholic FermentationBeer FermentationStrain ImprovementSulfur MetabolismYeast BiologyYeast GeneticsBeer ProductionHydrogen Sulfide MetabolismSaccharomyces Cerevisiae FermentationSulfur Compound Formation During FermentationSulfur Compound MetabolismSulfur Gene ExpressionSulfur Metabolism EnzymesSulfur-responsive Gene Expression
NHS5, a gene suppressing formation of hydrogen sulfide (H2S), was cloned from a Saccharomyces cerevisiae X2180-1A gene library. The gene was inserted into suitable vectors and introduced into bottom-fermentation yeasts. We verified that the strain with the inserted gene was effective in beer production because the gene reduced H2S content in green beer and did not affect other fermentation characters. We also proved that the function of the gene was related to production of cystathionine β-synthase.