Production and Purification of the Heavy Chain Fragment C of Botulinum Neurotoxin, Serotype A, Expressed in the Methylotrophic Yeast Pichia pastoris

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

Koti Sreekrishna, Robert G. Brankamp, Keith E. Kropp et al. · Gene · 1997 · 437 citations

Life Sciences, Biotechnology, Molecular Biology +16

Pichia pastoris as a host system for transformations.

J M Cregg, Kevin Barringer, Anita Y. Hessler et al. · Molecular and Cellular Biology · 1985 · 431 citations

Life Sciences, Genetics, Molecular Biology +17

Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E.

Giampietro Schiavo, Ornella Rossetto, S. Catsicas et al. · Journal of Biological Chemistry · 1993 · 428 citations · Full text

Medicine, Neurobiology, Neuroscience +14