Journal of the Science of Food and Agriculture · 1999 · 54 citations · 0 references
AgricultureBiochemistryFood ScienceCarbohydrate ChemistryFood AnalysisFood ChemistryCarbohydrate AnalysisCarbohydrate CharacterizationCarbohydrate StructureCarbohydrates In FoodsOrganic AcidsStarch ChemistryCarbohydrate Composition AnalysisFeedstuffs Carbohydrate CompositionOrganic Acids In FoodsPolysaccharide Fractionation
A method was developed to fractionate the neutral detergent-soluble carbohydrates (NDSC) in feedstuffs. Differential solubilities of carbohydrates in 80:20 (v/v) ethanol/water were used to partition NDSC into organic acids (OA) and mono- and oligosaccharides soluble in ethanol/water from starch and neutral detergent-soluble fibre (NDSF) which are insoluble. Mono- and oligosaccharides (total ethanol/water-soluble carbohydrate) were measured on the ethanol/water extract, and starch was measured on the ethanol/water-insoluble residue. The OA and NDSF, the two most compositionally diverse NDSC fractions, were estimated by difference. The method allows partitioning of the NDSC on a nutritionally relevant basis into (1) organic acids, (2) total ethanol/water-soluble carbohydrate, (3) starch and (4) neutral detergent-soluble fibre. The methods involved in this fractionation are relatively simple or are commonly used. © 1999 Society of Chemical Industry